Both males and females call, but only males call by inflating the throat sac. Size isn't an accurate measure either until the frogs are fully grown. Dark throats are another red herring, I have a female with a dark throat, and a male with a light throat, so that's nothing to go by.
I find the most accurate way to tell a young frog's sex is to look at the front feet for nuptial pads. They appear roughly the same time as the males start to call frequently, which is when they are about 2" (5cm) snout to vent length. To be sure, I'd leave it until they are 2.5" (6cm) SNV.
So, this is how you do it. The nuptial pad is a brown patch on the inside of the thumb of the male, which is totally absent from females. It's present all year round, though it's darker during the breeding season.
First of all, here is my big female
This is what her thumb looks like, no nuptial pad
Now here is a male, you can already tell because his throat sac is inflated
Here is his thumb
See the brown area at the base of his thumb? That tells us he is definately a male.
Now, here's a few more examples. Try to guess whether it's a male or female
Frog #3
Thumb shot
Frog #4
Thumb shot
Frog #5
Thumb shot
Incase you couldn't tell, frog #3 was female and frogs #4 and #5 were males
By the way, none of these frogs are fully mature, except maybe the first one there, but as you can see you can tell well before breeding age what sex your White's treefrog is.
Thank you very much and I hope this guide helped you
